Archived: VIRTUAL TRAINING EVENT | Biocatalysis – Solving Scale-Up Issues with Biocatalysed Reactions

Date: 15 July - 16 July 2020

Location: Online Event

Tutors: Dr Andrew Wells

This is a VIRTUAL short course will look at biocatalysed/biotransformation reactions to make pharma/agro chem intermediates, API’s and fine chemicals. the focus is on scale-up issues and getting productive process established.

July 15, 1.30 – 4.30 pm (UK)  |  Session 1

July 16, 1.30 – 4.30 (UK) |  Session 2

Many forays by organic chemists into using biocatalysis in chemical synthesis stall at the initial scale-up stage. Screening and small scale (gram) reactions progress as planned, but when scaled -up, reactions stall, take more enzyme than expected, work-ups end up with emulsion issues etc. This course will look at some of the techniques and technologies ( tricks of the trade) that can help derisk scaling up biocatalysed reactions .A further barrier to the adoption of more biocatalytic reactions are production / EHS scientists who are unsure how to handle enzymes in the plant/pilot plant. the course will also look at good sources of information/data to facilitate the use of enzymes at scale.

  • Enzyme supply chain: Storage, stability etc
  • Techno-economic analysis – will the transformation ever hit the required targets?
  • Different types of strains used and its safety impactions.
  • Using GMO’s and GMO derived materials
  • Handling concerns related to enzyme -SHE/EHS issues., Safety issues in scaling up biocatalytic reactions
  • Pros and cons of different enzyme formulations
  • Enzyme stability: General to pH, co-solvent, temperature as linked to process design and development
  • Enzyme analysis / characterisation – needed to assess purity in order to calculate activity and so expected long term cost, spec setting / quality control (ensure plant samples match lab samples and measure variation over time).
  • Scale-up – reactor options – various reactor types, std batch, continuous, membrane separated, continuous liquid extraction, etc
  • Product isolation from complex mix . Enzyme removal/ downstream processing– to be intelligent in terms of what can and can’t be removed vs effects on process including downstream chemistry.
  • Use of immobilised enzymes/ Recycling of immobilised enzymes
  • Some IP considerations

When you register you will be sent links to join the on-line sessions. These will take place at the following time each day:

1.30 – 4.30 pm  |  UK

2.30 – 5.30  |  Central Europe

08.30 – 11.30 |  Eastern US

5.30 – 8.30 am  |  West Coast US

As space is limited there is a small charge of £195.00 + VAT* per person (*only added if your company is based in the UK) Payments only available via credit card.

Who should attend?

This online class will be of interest to anyone who are:

Scientists working in medicinal/process chemistry and are interested in moving from mg/screening scale to 100’ s grams-multi kg scale

Please note: this course assumes some prior existing knowledge of biocatalysis / biotransformations as applied to synthetic organic chemistry, either in theory or some prior practical use at screening/ small scale laboratory experimentation, an appreciation of different enzyme classes etc.


Virtual Delegate 2020

I really enjoyed the virtual Short Course and it was very good incl. the thorough collection of examples etc.

Looking forward to the next ones!