Archived: Simulation of Chromatographic Processes in Biotechnology Industries

Date: 25 November - 26 November 2021

Location: Frankfurt, Germany

Tutors: Dr Lucrèce Nicoud, Dr Yohann LeGuennec

This course will present an overview of what simulation can bring for the development, optimization and troubleshooting of chromatographic processes. It will focus on applications relevant for biotech industries (e.g., buffers, amino acids, proteins, oligonucleotides). 

The course will be balanced between theoretical lectures and hands-on sessions. Theoretical lectures will provide the fundamentals of chromatography modeling, which derives from the understanding and description of the physical phenomena at stake, namely thermodynamics, kinetics of mass transfer and hydrodynamics. In the hands-on sessions, the attendees will have access to the software Ionic®. They will be able to play with the software and perform simulations on their own, in particular to predict the impact of key operating parameters (e.g., crude composition, loading volume, flowrate, gradient slope,…) on process performances.

The course will be presented through practical case studies covering various chromatography techniques: size exclusion, affinity, hydrophobic interaction and ion exchange chromatography. For each type of chromatography, a proper mechanistic model will be presented and the key experiments necessary to identify model parameters will be discussed.

Course Outline

DAY I

  • Basics 
  • A bit of chromatography history
  • Overview of the chromatography types
  • Key physical phenomena
  • What modeling can bring

 

  • Size Exclusion Chromatography 
  • Fluid partitioning and porosities
  • Column characterization with tracers
  • Hydrodynamics
  • Linear adsorption isotherms
  • Case study 1: tracers use
  • Case study 2: removal of protein aggregates

 

  • Affinity chromatography 
  • Langmuir adsorption isotherms
  • Mass transfer limitations
  • Multicolumn processes
  • Case study: mAb purification on Protein A 

DAY II

  • Hydrophobic Interaction chromatography
  • Aqueous solution chemistry
  • Calculation of technical performances
  • Basics of process optimization
  • Case study: protein purification

 

  • Ion exchange chromatography
  • Differences between adsorption and ion exchange
  • Basis of an ion exchange model
  • Case study 1: Na+/Ca2+separation 
  • Case study 2: importance of buffer selection 
  • Case study 3: Lysine purification
  • Case study 4: oligonucleotide purification 
  • Summary and Q&A

What's Included

The course fee includes:

  • comprehensive course manual
  • coffee/tea refreshments breaks throughout each day
  • buffet lunch each day
  • course dinner on the first evening
  • course certificate

Who should attend?

Experienced lab scientists and process engineers working in biotechnological industries interested in learning more on the use of mechanistic modelling for the development and optimization of chromatographic processes

Testimonials

Ipsen Biopharm

Very insightful and informative course. Thoroughly enjoyed all aspects, especially background equations and troubleshooting/case studies. Also enjoyed simulations using software as they are more visual.

2018 Delegate

Thank you very much, course helped me to deeper understand how SMB works! Time well spent!

2018 Delegate

Very competent, flexible and open presenters.

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Archived: Simulation of Chromatographic Processes in Biotechnology Industries

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