Simulation of Chromatographic Processes in Biotechnology Industries
Event overview
This course will present an overview of what simulation can bring for the development, optimization and troubleshooting of chromatographic processes. It will focus on applications relevant for biotech industries (e.g., buffers, amino acids, proteins, oligonucleotides).
The course will be balanced between theoretical lectures and hands-on sessions. Theoretical lectures will provide the fundamentals of chromatography modeling, which derives from the understanding and description of the physical phenomena at stake, namely thermodynamics, kinetics of mass transfer and hydrodynamics. In the hands-on sessions, the attendees will have access to the software Ionic®. They will be able to play with the software and perform simulations on their own, in particular to predict the impact of key operating parameters (e.g., crude composition, loading volume, flowrate, gradient slope,…) on process performances.
The course will be presented through practical case studies covering various chromatography techniques: size exclusion, affinity, hydrophobic interaction and ion exchange chromatography. For each type of chromatography, a proper mechanistic model will be presented and the key experiments necessary to identify model parameters will be discussed.
Course Outline
DAY I
- Basics
- A bit of chromatography history
- Overview of the chromatography types
- Key physical phenomena
- What modeling can bring
- Size Exclusion Chromatography
- Fluid partitioning and porosities
- Column characterization with tracers
- Hydrodynamics
- Linear adsorption isotherms
- Case study 1: tracers use
- Case study 2: removal of protein aggregates
- Affinity chromatography
- Langmuir adsorption isotherms
- Mass transfer limitations
- Multicolumn processes
- Case study: mAb purification on Protein A
DAY II
- Hydrophobic Interaction chromatography
- Aqueous solution chemistry
- Calculation of technical performances
- Basics of process optimization
- Case study: protein purification
- Ion exchange chromatography
- Differences between adsorption and ion exchange
- Basis of an ion exchange model
- Case study 1: Na+/Ca2+separation
- Case study 2: importance of buffer selection
- Case study 3: Lysine purification
- Case study 4: oligonucleotide purification
- Summary and Q&A
What's Included
The course fee includes:
- comprehensive course manual
- coffee/tea refreshments breaks throughout each day
- buffet lunch each day
- course dinner on the first evening
- course certificate
Who should attend?
Experienced lab scientists and process engineers working in biotechnological industries interested in learning more on the use of mechanistic modelling for the development and optimization of chromatographic processes
Other Information
General Information
Each course includes:
- Refreshments & lunch daily
- Dinner on the first evening*
- The comprehensive Course Manual
- Course Certificate
Please refer to the course brochure for detailed timings.
*Not every course includes dinner please refer to the course brochures for detailed information on what’s included.
Course Certificate
At the end of each course participants receive a Certificate of Attendance – signed by your tutor.
Accommodation
Depending on venues we either reserve a limited number of bedrooms at a special rate for you or we will provide a list of recommended hotels close by to the course venue. More information on rates and booking forms will be provided upon booking. All rooms will need to booked directly with the hotel.
Discounts
1st delegate = full price
2nd delegate = 5% off
3rd delegate = 10% off
4 or more delegates = 15% off
For more information on discounts do give us a call on 44 1435 873 062
Venue info
60329
Frankfurt
Germany
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