Designing Reliable ION-EXCHANGE Chromatographic Processes with a Proven Predictive Approach

Date: 03 November - 04 November 2020

Location: Basel, Switzerland

Tutors: Dr Hector Osuna, Dr Lucrèce Nicoud


This course will present an overview of the state of the art in ion-exchange process chromatography: development, scale-up and optimization of preparative chromatographic processes. The attendees will learn how to develop ion-exchange preparative chromatographic processes. They will gain knowledge on the scale-up and optimization of ion-exchange processes.

The course will cover the fundamentals of process chromatography and the tutors will present a rational and proven methodology for the design of efficient and robust chromatographic processes. They will show how this approach serves both the design of new processes and the resolution of issues encountered in the daily life of chromatographers concerned by process development and scale-up. The methodology rests upon the rigorous description of the physical phenomena at stake, which includes thermodynamics, kinetics of mass transfer and hydrodynamics of the separation. Predictive models are derived from the understanding of these phenomena and are then used to assess the impact of operating parameters on the process performances. A focus will be done on ion-exchange chromatography and main features of the thermodynamic model.

Major applications of ion-exchange chromatography will also be presented, spanning across the ton-scale amino-acids or organic acids production, sugar softening, metals separation or the purification of proteins with stringent purity constraints. Various case studies inspired from real industrial examples or taken from the most recent literature will illustrate that the general approach presented during these two day applies as a roadmap, irrespective of the application, and ultimately leads to the development of reliable and robust processes.

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Course Outline


Day 1


Generalities and definition
A bit of history
Introduction to modeling

Operating modes
Multi-column applications


Fluid partitioning
Inert tracers

Equilibria (Thermodynamics)

Equilibria in solution
Liquid-solid Equilibria

Band broadening
The MC/PD models

Mass transfer (kinetics)
Basic considerations
Some notions about diffusion
The Fick’s law at a glance
The LDF approximation
Van Deemter equation, comparison of equilibrium and LDF models

The autopsy of a model

List of model parameters
Impact of the parameters on the chromatograms
Orders of magnitude and rigorous estimation

Day 2

III. Design

Generalities on scale-up and economics

Cost function

Single-column Chromatography

Case study

Counter-current multicolumn processes

Fundamentals of countercurrent
Basic design and shortcut
Case studies

Daily Life

Issues identification


Take home message and conclusions


Q&A discussion

Who the course is aimed at?

The course is designed for (Bio)Process Engineers, Scientists and Managers concerned by the downstream in the fields of fine chemicals, metals, pharma, biopharma, industrial biotechnology and bioprocessing.

What's Included

The course fee includes:

  • comprehensive course manual
  • coffee/tea refreshments breaks throughout each day
  • buffet lunch each day
  • course dinner on the first evening
  • course certificate

Upcoming Webinar


Date: 20 May 2020

Time: 15:00 (GMT), 16.00 (CET), 10.00 (ET), 7.00 (PT)

Tutors: Dr Hector Osuna